Identical sets of methylated and nonmethylated genes in Ciona intestinalis sperm and muscle cells.

ABSTRACT:

Background

The discovery of gene body methylation, which refers to DNA methylation within gene coding region, suggests an as yet unknown role of DNA methylation at actively transcribed genes. In invertebrates, gene bodies are the primary targets of DNA methylation, and only a subset of expressed genes is modified.

Results

Here we investigate the tissue variability of both the global levels and distribution of 5-methylcytosine (5mC) in the sea squirt Ciona intestinalis. We find that global 5mC content of early developmental embryos is high, but is strikingly reduced in body wall tissues. We chose sperm and adult muscle cells, with high and reduced levels of global 5mC respectively, for genome-wide analysis of 5mC targets. By means of CXXC-affinity purification followed by dee

SUBMITTER: Suzuki MM 

PROVIDER: S-EPMC3827831 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature