Construction of a full-length infectious bacterial artificial chromosome clone of duck enteritis virus vaccine strain.

ABSTRACT:

Background

Duck enteritis virus (DEV) is the causative agent of duck viral enteritis, which causes an acute, contagious and lethal disease of many species of waterfowl within the order Anseriformes. In recent years, two laboratories have reported on the successful construction of DEV infectious clones in viral vectors to express exogenous genes. The clones obtained were either created with deletion of viral genes and based on highly virulent strains or were constructed using a traditional overlapping fosmid DNA system. Here, we report the construction of a full-length infectious clone of DEV vaccine strain that was cloned into a bacterial artificial chromosome (BAC).

Methods

A mini-F vector as a BAC that allows the maintenance of large circular DNA in E. coli was introduced

SUBMITTER: Chen L 

PROVIDER: S-EPMC3827880 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature