A novel multigene expression construct for modification of glycerol metabolism in Yarrowia lipolytica.

ABSTRACT:

Background

High supply of raw, residual glycerol from biodiesel production plants promote the search for novel biotechnological methods of its utilization. In this study we attempted modification of glycerol catabolism in a nonconventional yeast species Yarrowia lipolytica through genetic engineering approach.

Results

To address this, we developed a novel genetic construct which allows transferring three heterologous genes, encoding glycerol dehydratase, its reactivator and a wide-spectrum alcohol oxidoreductase under the control of glycerol-induced promoter. The three genes, tandemly arrayed in an expression cassette with a marker gene ura3, regulatory and targeting sequences (G3P dh promoter and XPR-like terminator, 28S rDNA as a target locus), were transferred into Yarrow

SUBMITTER: Celinska E 

PROVIDER: S-EPMC3827991 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature