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T cell activation is determined by the number of presented antigens.

ABSTRACT: Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90-140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm(2). We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented pMHCs dominates over local pMHC density.

SUBMITTER: Deeg J

PROVIDER: S-EPMC3828117 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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T cell activation is determined by the number of presented antigens.

Deeg Janosch J Axmann Markus M Matic Jovana J Liapis Anastasia A Depoil David D Afrose Jehan J Curado Silvia S Dustin Michael L ML Spatz Joachim P JP

Nano letters 20131017 11

Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90-140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm(2). We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented p ...[more]

PMID: 24117051

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