Receptor concentration and diffusivity control multivalent binding of Sv40 to membrane bilayers.

ABSTRACT: Incoming Simian Virus 40 particles bind to their cellular receptor, the glycolipid GM1, in the plasma membrane and thereby induce membrane deformation beneath the virion leading to endocytosis and infection. Efficient membrane deformation depends on receptor lipid structure and the organization of binding sites on the internalizing particle. To determine the role of receptor diffusion, concentration and the number of receptors required for stable binding in this interaction, we analyze the binding of SV40 to GM1 in supported membrane bilayers by computational modeling based on experimental data. We measure the diffusion rates of SV40 virions in solution by fluorescence correlation spectroscopy and of the receptor in bilayers by single molecule tracking. Quartz-crystal microbalance with dis

SUBMITTER: Szklarczyk OM 

PROVIDER: S-EPMC3828148 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature