ShRNA-mediated Slc38a1 silencing inhibits migration, but not invasiveness of human pancreatic cancer cells.

ABSTRACT:

Objective

Early metastasis is a major biological feature of pancreatic cancer. The current study examined whether silencing Slc38a1, a gene involved in energy metabolism, using short hairpin RNA (shRNA) could inhibit the growth, migration, and invasiveness of pancreatic cancer cells.

Methods

A series of Slc38a1 shRNAs were designed and cloned into the pGPU6/GFP/Neo vectors. An shRNA with the most efficacious inhibitory action on SCL38A1 expression (65% inhibition) upon screening in DH5α bacteria was used to transfect SW1990 human pancreatic cancer cells. Cell growth, migration, and invasiveness were examined using cell counting kit-8, Boyden chamber without and with Matrigel, respectively.

Results

Transfection of SW1990 cells with the SLCs38A1 shRNA significantly decr

SUBMITTER: Xie J 

PROVIDER: S-EPMC3828439 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature