Human endogenous retroviral syncytin exerts inhibitory effect on invasive phenotype of B16F10 melanoma cells.

ABSTRACT:

Objective

Fusogenic endogenous retroviral syncytin plays an important role in the formation of syncytiotrophoblasts in human placenta. Apart from its expression in placenta, brain and testis, syncytin has also been found in many cancers. Although syncytin has been proposed to serve as a positive prognostic marker in some cancers, the underlying mechanism is unclear. The aim of this study is to evaluate the effects of syncytin expression on the invasive phenotype of melanoma cells.

Methods

The eukaryotic expression plasmid for syncytin-EGFP was constructed and transfected into B16F10 melanoma cells. The effect of syncytin on the invasion potential of tumor cells was evaluated in B16F10 subline cells that stably expressed syncytin-EGFP fusion protein or EGFP alone.

Results<

SUBMITTER: Mo H 

PROVIDER: S-EPMC3828447 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature