Membrane association of a protein increases the rate, extent, and specificity of chemical cross-linking.

ABSTRACT: Many cellular processes involve interactions between membrane-associated proteins, and those interactions are enhanced by membrane association. We have used cross-linking reactions to compare the extent and specificity of protein interactions in solution versus on a membrane surface. Cysteine mutants of a soluble cytoplasmic fragment (CF) of the aspartate receptor, a transmembrane receptor involved in bacterial chemotaxis, are used in disulfide bond formation with the thiol-specific oxidant diamide and chemical cross-linking reactions with the trifunctional maleimide TMEA. CF binding to membranes is mediated by its N-terminal His tag binding to vesicles containing a nickel-chelating lipid, so cross-linking reactions conducted in the presence and absence of vesicles differ only in whether C

SUBMITTER: Mudiyanselage AP 

PROVIDER: S-EPMC3830642 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature