Role of endothelial N-glycan mannose residues in monocyte recruitment during atherogenesis.

ABSTRACT:

Objective

Upregulated expression of endothelial adhesion molecules and subsequent binding to cognate monocytic receptors are established paradigms in atherosclerosis. However, these proteins are the scaffolds, with their posttranslational modification with sugars providing the actual ligands. We recently showed that tumor necrosis factor-α increased hypoglycosylated (mannose-rich) N-glycans on the endothelial surface. In the present study, our aim was to determine whether (1) hypoglycosylated N-glycans are upregulated by proatherogenic stimuli (oscillatory flow) in vitro and in vivo, and (2) mannose residues on hypoglycosylated endothelial N-glycans mediate monocyte rolling and adhesion.

Methods and results

Staining with the mannose-specific lectins concanavalin A and lens c

SUBMITTER: Scott DW 

PROVIDER: S-EPMC3831355 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature