Identification of Streptococcus thermophilus CNRZ368 genes involved in defense against superoxide stress.

ABSTRACT: To better understand the defense mechanism of Streptococcus thermophilus against superoxide stress, molecular analysis of 10 menadione-sensitive mutants, obtained by insertional mutagenesis, was undertaken. This analysis allowed the identification of 10 genes that, with respect to their putative functions, were classified into five categories: (i) those involved in cell wall metabolism, (ii) those involved in exopolysaccharide translocation, (iii) those involved in RNA modification, (iv) those involved in iron homeostasis, and (v) those whose functions are still unknown. The behavior of the 10 menadione-sensitive mutants exposed to heat shock was investigated. Data from these experiments allowed us to distinguish genes whose action might be specific to oxidative stress defense (tgt, ossF,

SUBMITTER: Thibessard A 

PROVIDER: S-EPMC383142 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature