Three-dimensional reconstruction of icosahedral particles from single micrographs in real time at the microscope.

ABSTRACT: Single particle analysis is a valuable tool in cryo-electron microscopy for determining the structure of biological complexes. However, the conformational state and the preparation of the sample are factors that play a critical role in the ultimate attainable resolution. In some cases extensive analysis at the microscope of a sample under different conditions is required to derive the optimal acquisition conditions. Currently this analysis is limited to raw micrographs, thus conveying only limited information on the structure of the complex. We are developing a computing system that generates a three-dimensional reconstruction from a single micrograph acquired under cryogenic and low dose conditions, and containing particles with icosahedral symmetry. The system provides the microscopist w

SUBMITTER: Cardone G 

PROVIDER: S-EPMC3831522 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature