Validation of doubled haploid plants by enzymatic mismatch cleavage.

ABSTRACT:

Background

Doubled haploidy is a fundamental tool in plant breeding as it provides the fastest way to generate populations of meiotic recombinants in a genetically fixed state. A wide range of methods has been developed to produce doubled haploid (DH) plants and recent advances promise efficient DH production in otherwise recalcitrant species. Since the cellular origin of the plants produced is not always certain, rapid screening techniques are needed to validate that the produced individuals are indeed homozygous and genetically distinct from each other. Ideal methods are easily implemented across species and in crops where whole genome sequence and marker resources are limited.

Results

We have adapted enzymatic mismatch cleavage techniques commonly used for TILLING (Target

SUBMITTER: Hofinger BJ 

PROVIDER: S-EPMC3831592 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature