Global analysis of target genes of 21 members of the ZAD transcription factor family in Drosophila melanogaster.

ABSTRACT: The zinc-finger associated domain (ZAD) family is the largest transcription factor family in dipteran insects. Still, their functional significance is barely recognized in the literature due in part to their resistance to mutagenesis screens in genetic studies. Therefore, we employed in vitro techniques to identify the DNA-binding characteristics of several members of the Drosophila melanogaster ZAD family in an effort to study their target genes. In this comprehensive investigation, we constructed a panel of GST-Zinc finger (ZnF) array chimera from 21 selected ZAD proteins and used them to select binding sites from an oligonucleotide library by employing electrophoretic mobility shift assays (EMSA). Samples of the binding population were sequenced and used to derive DNA-binding consensus

SUBMITTER: Krystel J 

PROVIDER: S-EPMC3831651 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature