Differential expression of olfactory genes in the southern house mosquito and insights into unique odorant receptor gene isoforms.

ABSTRACT: The southern house mosquito, Culex quinquefasciatus, has one of the most acute and eclectic olfactory systems of all mosquito species hitherto studied. Here, we used Illumina sequencing to identify olfactory genes expressed predominantly in antenna, mosquito's main olfactory organ. Less than 50% of the trimmed reads generated by high-quality libraries aligned to a transcript, but approximately 70% of them aligned to the genome. Differential expression analysis, which was validated by quantitative real-time PCR on a subset of genes, showed that approximately half of the 48 odorant-binding protein genes were enriched in antennae, with the other half being predominantly expressed in legs. Similar patterns were observed with chemosensory proteins, "plus-C" odorant-binding proteins, and sensory

SUBMITTER: Leal WS 

PROVIDER: S-EPMC3831946 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature