Yeast metabolic and signaling genes are required for heat-shock survival and have little overlap with the heat-induced genes.

ABSTRACT: Genome-wide gene-expression studies have shown that hundreds of yeast genes are induced or repressed transiently by changes in temperature; many are annotated to stress response on this basis. To obtain a genome-scale assessment of which genes are functionally important for innate and/or acquired thermotolerance, we combined the use of a barcoded pool of ~4,800 nonessential, prototrophic Saccharomyces cerevisiae deletion strains with Illumina-based deep-sequencing technology. As reported in other recent studies that have used deletion mutants to study stress responses, we observed that gene deletions resulting in the highest thermosensitivity generally are not the same as those transcriptionally induced in response to heat stress. Functional analysis of identified genes revealed that metab

SUBMITTER: Gibney PA 

PROVIDER: S-EPMC3831991 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature