Links between the oncoprotein YB-1 and small non-coding RNAs in breast cancer.

ABSTRACT:

Background

The nucleic acid-binding protein YB-1, a member of the cold-shock domain protein family, has been implicated in the progression of breast cancer and is associated with poor patient survival. YB-1 has sequence similarity to LIN28, another cold-shock protein family member, which has a role in the regulation of small noncoding RNAs (sncRNAs) including microRNAs (miRNAs). Therefore, to investigate whether there is an association between YB-1 and sncRNAs in breast cancer, we investigated whether sncRNAs were bound by YB-1 in two breast cancer cell lines (luminal A-like and basal cell-like), and whether the abundance of sncRNAs and mRNAs changed in response to experimental reduction of YB-1 expression.

Results

RNA-immunoprecipitation with an anti-YB-1 antibody showed th

SUBMITTER: Blenkiron C 

PROVIDER: S-EPMC3832415 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature