Microelectrode array biosensor for studying carbohydrate-mediated interactions.

ABSTRACT: Carbohydrate-mediated host-pathogen interactions are essential to bacterial and viral pathogenesis, and represent an attractive target for the development of antiadhesives to prevent infection. We present a versatile microelectrode array-based platform to investigate carbohydrate-mediated protein and bacterial binding, with the objective of developing a generalizable method for screening inhibitors of host-microbe interactions. Microelectrode arrays are well suited for interrogating biological binding events, including proteins and whole-cells, and are amenable to electrochemical derivitization, facilitating rapid deposition of biomolecules. In this study, we achieve microelectrode functionalization with carbohydrates via controlled polymerization of pyrrole to individual microelectrodes,

SUBMITTER: Chamberlain JW 

PROVIDER: S-EPMC3832429 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature