Generation of doubled haploid transgenic wheat lines by microspore transformation.

ABSTRACT: Microspores can be induced to develop homozygous doubled haploid plants in a single generation. In the present experiments androgenic microspores of wheat have been genetically transformed and developed into mature homozygous transgenic plants. Two different transformation techniques were investigated, one employing electroporation and the other co-cultivation with Agrobacterium tumefaciens. Different tissue culture and transfection conditions were tested on nine different wheat cultivars using four different constructs. A total of 19 fertile transformants in five genotypes from four market classes of common wheat were recovered by the two procedures. PCR followed by DNA sequencing of the products, Southern blot analyses and bio/histo-chemical and histological assays of the recombinant enz

SUBMITTER: Brew-Appiah RA 

PROVIDER: S-EPMC3832437 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature