The approach to sample acquisition and its impact on the derived human fecal microbiome and VOC metabolome.

ABSTRACT: Recent studies have illustrated the importance of the microbiota in maintaining a healthy state, as well as promoting disease states. The intestinal microbiota exerts its effects primarily through its metabolites, and metabolomics investigations have begun to evaluate the diagnostic and health implications of volatile organic compounds (VOCs) isolated from human feces, enabled by specialized sampling methods such as headspace solid-phase microextraction (hSPME). The approach to stool sample collection is an important consideration that could potentially introduce bias and affect the outcome of a fecal metagenomic and metabolomic investigation. To address this concern, a comparison of endoscopically collected (in vivo) and home collected (ex vivo) fecal samples was performed, revealing slig

SUBMITTER: Couch RD 

PROVIDER: S-EPMC3832442 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature