Transcriptome sequencing and microarray design for functional genomics in the extremophile Arabidopsis relative Thellungiella salsuginea (Eutrema salsugineum).

ABSTRACT:

Background

Most molecular studies of plant stress tolerance have been performed with Arabidopsis thaliana, although it is not particularly stress tolerant and may lack protective mechanisms required to survive extreme environmental conditions. Thellungiella salsuginea has attracted interest as an alternative plant model species with high tolerance of various abiotic stresses. While the T. salsuginea genome has recently been sequenced, its annotation is still incomplete and transcriptomic information is scarce. In addition, functional genomics investigations in this species are severely hampered by a lack of affordable tools for genome-wide gene expression studies.

Results

Here, we report the results of Thellungiella de novo transcriptome assembly and annotation based on 454

SUBMITTER: Lee YP 

PROVIDER: S-EPMC3832907 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature