Genetic analysis of structural proteins in the adsorption apparatus of bacteriophage epsilon 15.

ABSTRACT:

Aim

To probe the organizational structure of the adsorption apparatus of bacteriophage epsilon 15 (E15) using genetic and biochemical methodology

Methods

Hydroxylamine was used to create nonsense mutants of bacteriophage E15. The mutants were then screened for defects in their adsorption apparatus proteins, initially by measuring the concentrations of free tail spike proteins in lysates of cells that had been infected by the phage mutants under non-permissive growth conditions. Phage strains whose infected cell lysates contained above-average levels of free tail spike protein under non-permissive growth conditions were assumed to contain nonsense mutations in genes coding for adsorption apparatus proteins. These mutants were characterized by classical genetic mapping methods

SUBMITTER: Guichard JA 

PROVIDER: S-EPMC3832910 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature