Multiparametric image analysis of lung-branching morphogenesis.

ABSTRACT:

Background

Lung-branching morphogenesis is a fundamental developmental process, yet the cellular dynamics that occur during lung development and the molecular mechanisms underlying recent postulated branching modes are poorly understood.

Results

Here, we implemented a time-lapse video microscopy method to study the cellular behavior and molecular mechanisms of planar bifurcation and domain branching in lung explant- and organotypic cultures. Our analysis revealed morphologically distinct stages that are shaped at least in part by a combination of localized and orientated cell divisions and by local mechanical forces. We also identified myosin light-chain kinase as an important regulator of bud bifurcation, but not domain branching in lung explants.

Conclusions

This li

SUBMITTER: Schnatwinkel C 

PROVIDER: S-EPMC3833092 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature