Preclinical validation of Aurora kinases-targeting drugs in osteosarcoma.

ABSTRACT:

Background

Aurora kinases are key regulators of cell cycle and represent new promising therapeutic targets in several human tumours.

Methods

Biological relevance of Aurora kinase-A and -B was assessed on osteosarcoma clinical samples and by silencing these genes with specific siRNA in three human osteosarcoma cell lines. In vitro efficacy of two Aurora kinases-targeting drugs (VX-680 and ZM447439) was evaluated on a panel of four drug-sensitive and six drug-resistant human osteosarcoma cell lines.

Results

Human osteosarcoma cell lines proved to be highly sensitive to both drugs. A decreased drug sensitivity was observed in doxorubicin-resistant cell lines, most probably related to ABCB1/MDR1 overexpression. Both drugs variably induced hyperploidy and apoptosis in the

SUBMITTER: Tavanti E 

PROVIDER: S-EPMC3833226 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature