The broccoli (Brassica oleracea) phloem tissue proteome.

ABSTRACT:

Background

The transport of sugars, hormones, amino acids, proteins, sugar alcohols, and other organic compounds from the sites of synthesis to the sites of use or storage occurs through the conducting cells of the phloem. To better understand these processes a comprehensive understanding of the proteins involved is required. While a considerable amount of data has been obtained from proteomic analyses of phloem sap, this has mainly served to identify the soluble proteins that are translocated through the phloem network.

Results

In order to obtain more comprehensive proteomic data from phloem tissue we developed a simple dissection procedure to isolate phloem tissue from Brassica oleracea. The presence of a high density of phloem sieve elements was confirmed using light micr

SUBMITTER: Anstead JA 

PROVIDER: S-EPMC3833381 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature