Assessing the influence of adsorbed-state conformation on the bioactivity of adsorbed enzyme layers.

ABSTRACT: Systems using immobilized enzymes are attractive for a wide range of industrial and medical applications because they allow for the fabrication of stable, reusable substrates with highly specific functionality. The performance of these systems is greatly dependent upon the orientation and conformation of the adsorbed enzymes. To investigate these relationships, we have developed and applied methods to quantitatively assess the secondary structure of adsorbed enzyme layers on planar surfaces using circular dichroism (CD) spectroscopy and evaluate their bioactivity using colorimetric assays. These combined measurements provide molecular-level insights regarding whether observed changes in adsorbed enzyme bioactivity are due to the adsorbed orientation of an enzyme or adsorption-induced chang

SUBMITTER: Fears KP 

PROVIDER: S-EPMC3833468 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature