Effect of gestational age on mRNA and protein expression of polyspecific organic cation transporters during pregnancy.

ABSTRACT: Polyspecific organic cation (OC) transporters play important roles in the disposition of clinically used drugs, including drugs used during pregnancy. Pregnancy is known to alter the expression of drug-metabolizing enzymes and transporters, but its specific effect on OC transporters has not been well defined. Using quantitative polymerase chain reaction and liquid chromatography coupled with tandem mass spectrometry targeted proteomics, we determined the effect of pregnancy and gestational age on mRNA and protein expression of major OC transporters in the kidney, liver, and placenta in mice with timed pregnancies. Human organic cation transporter 3 (hOCT3) expression was further investigated in human placentas from the first and second trimesters and at term. Our results showed that pregna

SUBMITTER: Lee N 

PROVIDER: S-EPMC3834131 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature