Integrated profiling of three dimensional cell culture models and 3D microscopy.

ABSTRACT:

Motivation

Our goal is to develop a screening platform for quantitative profiling of colony organizations in 3D cell culture models. The 3D cell culture models, which are also imaged in 3D, are functional assays that mimic the in vivo characteristics of the tissue architecture more faithfully than the 2D cultures. However, they also introduce significant computational challenges, with the main barriers being the effects of growth conditions, fixations and inherent complexities in segmentation that need to be resolved in the 3D volume.

Results

A segmentation strategy has been developed to delineate each nucleus in a colony that overcomes (i) the effects of growth conditions, (ii) variations in chromatin distribution and (iii) ambiguities formed by perceptual boundaries from a

SUBMITTER: Bilgin CC 

PROVIDER: S-EPMC3834800 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature