Two- and three-input TALE-based AND logic computation in embryonic stem cells.

ABSTRACT: Biological computing circuits can enhance our ability to control cellular functions and have potential applications in tissue engineering and medical treatments. Transcriptional activator-like effectors (TALEs) represent attractive components of synthetic gene regulatory circuits, as they can be designed de novo to target a given DNA sequence. We here demonstrate that TALEs can perform Boolean logic computation in mammalian cells. Using a split-intein protein-splicing strategy, we show that a functional TALE can be reconstituted from two inactive parts, thus generating two-input AND logic computation. We further demonstrate three-piece intein splicing in mammalian cells and use it to perform three-input AND computation. Using methods for random as well as targeted insertion of these relati

SUBMITTER: Lienert F 

PROVIDER: S-EPMC3834826 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature