Homology-mediated end-capping as a primary step of sister chromatid fusion in the breakage-fusion-bridge cycles.

ABSTRACT: Breakage-fusion-bridge (BFB) cycle is a series of chromosome breaks and duplications that could lead to the increased copy number of a genomic segment (gene amplification). A critical step of BFB cycles leading to gene amplification is a palindromic fusion of sister chromatids following the rupture of a dicentric chromosome during mitosis. It is currently unknown how sister chromatid fusion is produced from a mitotic break. To delineate the process, we took an integrated genomic, cytogenetic and molecular approach for the recurrent MCL1 amplicon at chromosome 1 in human tumor cells. A newly developed next-generation sequencing-based approach identified a cluster of palindromic fusions within the amplicon at ∼50-kb intervals, indicating a series of breaks and fusions by BFB cycles. The phys

SUBMITTER: Marotta M 

PROVIDER: S-EPMC3834830 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature