Development of a site-directed integration plasmid for heterologous gene expression in Mycoplasma gallisepticum.

ABSTRACT: Deciphering the molecular basis of the interactions between the parasite Mycoplasma gallisepticum and its avian hosts suffers from the lack of genetic tools available for the pathogen. In the absence of well established methods for targeted disruption of relevant M. gallisepticum genes, we started to develop suicide vectors and equipped them with a short fragment of M. gallisepticum origin or replication (oriC MG). We failed to create a disruption vector, although by adding a further short fragment of the M. gallisepticum tufB upstream region we created a "Trojan horse" plasmid. This is fully integrated into the genomic DNA of M. gallisepticum, always at the same site, oriC MG, and is able to carry and express any gene of interest in the genetic background of M. gallisepticum. Successful e

SUBMITTER: Nieszner I 

PROVIDER: S-EPMC3835672 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature