Targeted glycomics by selected reaction monitoring for highly sensitive glycan compositional analysis.

ABSTRACT: The development of glycomics increasingly requires the detection and quantification of large numbers of glycans, which is only partially achieved by current glycomics approaches. Taking advantage of selected reaction monitoring to enhance both sensitivity and selectivity, we report here a strategy termed targeted glycomics that enables highly sensitive and consistent identification and quantification of diverse glycans across multiple samples at the same time. In this proof-of-principle study, we validated the method by analyzing global N-glycans expressed in different systems: single proteins, cancer cells, and serum samples. A dynamic range of three orders of magnitude was obtained for the detection of all five glycans released from ribonuclease B. The limit of detection of 80 attomole f

SUBMITTER: Zhang H 

PROVIDER: S-EPMC3836389 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature