Effects of culture and transplantation on follicle activation and early follicular growth in neonatal mouse ovaries.

ABSTRACT: Mouse models have been widely utilized to elucidate the basic principles and regulatory mechanisms of primordial follicle activation. Outside their natural environment, the growth of follicles might be affected by unknown factors in vitro and the elimination of regulation in vivo. Currently, in vitro culture and transplantation of ovaries under the kidney capsule are two commonly used incubation methods. However, the limited number of studies that have been published compare various incubation systems and reveal differences between ovaries that are incubated and grown in vivo. We compare the number of primordial, primary and secondary follicles in cultured, transplanted and in-vivo-grown ovaries. We investigate the expression levels of four genes, including zona pellucida 3 (ZP3), growth a

SUBMITTER: Wang S 

PROVIDER: S-EPMC3836445 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature