RADtyping: an integrated package for accurate de novo codominant and dominant RAD genotyping in mapping populations.

ABSTRACT: Genetic linkage maps are indispensable tools in genetic, genomic and breeding studies. As one of genotyping-by-sequencing methods, RAD-Seq (restriction-site associated DNA sequencing) has gained particular popularity for construction of high-density linkage maps. Current RAD analytical tools are being predominantly used for typing codominant markers. However, no genotyping algorithm has been developed for dominant markers (resulting from recognition site disruption). Given their abundance in eukaryotic genomes, utilization of dominant markers would greatly diminish the extensive sequencing effort required for large-scale marker development. In this study, we established, for the first time, a novel statistical framework for de novo dominant genotyping in mapping populations. An integrated

SUBMITTER: Fu X 

PROVIDER: S-EPMC3836964 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature