Polyamines are critical for the induction of the glutamate decarboxylase-dependent acid resistance system in Escherichia coli.

ABSTRACT: As part of our studies on the biological functions of polyamines, we have used a mutant of Escherichia coli that lacks all the genes for polyamine biosynthesis for a global transcriptional analysis on the effect of added polyamines. The most striking early response to the polyamine addition is the increased expression of the genes for the glutamate-dependent acid resistance system (GDAR) that is important for the survival of the bacteria when passing through the acid environment of the stomach. Not only were the two genes for glutamate decarboxylases (gadA and gadB) and the gene for glutamate-γ-aminobutyrate antiporter (gadC) induced by the polyamine addition, but the various genes involved in the regulation of this system were also induced. We confirmed the importance of polyamines for th

SUBMITTER: Chattopadhyay MK 

PROVIDER: S-EPMC3837104 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature