Roles of beta-tubulin residues Ala428 and Thr429 in microtubule formation in vivo.

ABSTRACT: The C termini of beta-tubulin isotypes are regions of high sequence variability that bind to microtubule-associated proteins and motors and undergo various post-translational modifications such as polyglutamylation and polyglycylation. Crystallographic analyses have been unsuccessful in resolving tubulin C termini. Here, we used a stepwise approach to study the role of this region in microtubule assembly. We generated a series of truncation mutants of human betaI and betaIII tubulin. Transient transfection of HeLa cells with the mutants shows that mutants with deletions of up to 22 residues from betaIII and 16 from betaI can assemble normally. Interestingly, removal of the next residue (Ala(428)) results in a complete loss of microtubule formation without affecting dimer formation. C-termi

SUBMITTER: Joe PA 

PROVIDER: S-EPMC3837401 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature