De novo assembly and characterization of the global transcriptome for Rhyacionia leptotubula using Illumina paired-end sequencing.

ABSTRACT:

Background

The pine tip moth, Rhyacionia leptotubula (Lepidoptera: Tortricidae) is one of the most destructive forestry pests in Yunnan Province, China. Despite its importance, less is known regarding all aspects of this pest. Understanding the genetic information of it is essential for exploring the specific traits at the molecular level. Thus, we here sequenced the transcriptome of R. leptotubula with high-throughput Illumina sequencing.

Methodology/principal findings

In a single run, more than 60 million sequencing reads were generated. De novo assembling was performed to generate a collection of 46,910 unigenes with mean length of 642 bp. Based on Blastx search with an E-value cut-off of 10(-5), 22,581 unigenes showed significant similarities to known proteins from Natio

SUBMITTER: Zhu JY 

PROVIDER: S-EPMC3837686 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature