Development of a tetracycline-inducible gene expression system for the study of Helicobacter pylori pathogenesis.

ABSTRACT: Deletion mutants and animal models have been instrumental in the study of Helicobacter pylori pathogenesis. Conditional mutants, however, would enable the study of the temporal gene requirement during H. pylori colonization and chronic infection. To achieve this goal, we adapted the Escherichia coli Tn10-derived tetracycline-inducible expression system for use in H. pylori. The ureA promoter was modified by inserting one or two tet operators to generate tetracycline-responsive promoters, named uPtetO, and these promoters were then fused to the reporter gfpmut2 and inserted into different loci. The expression of the tetracycline repressor (tetR) was placed under the control of one of three promoters and inserted into the chromosome. Conditional expression of green fluorescent protein (GFP)

SUBMITTER: Debowski AW 

PROVIDER: S-EPMC3837770 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature