Abi is required for modulation and stability but not localization or activation of the SCAR/WAVE complex.

ABSTRACT: The SCAR/WAVE complex drives actin-based protrusion, cell migration, and cell separation during cytokinesis. However, the contribution of the individual complex members to the activity of the whole remains a mystery. This is primarily because complex members depend on one another for stability, which limits the scope for experimental manipulation. Several studies suggest that Abi, a relatively small complex member, connects signaling to SCAR/WAVE complex localization and activation through its polyproline C-terminal tail. We generated a deletion series of the Dictyostelium discoideum Abi to investigate its exact role in regulation of the SCAR complex and identified a minimal fragment that would stabilize the complex. Surprisingly, loss of either the N terminus of Abi or the C-terminal poly

SUBMITTER: Davidson AJ 

PROVIDER: S-EPMC3837927 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature