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Molecular diagnosis of Actinomadura madurae infection by 16S rRNA deep sequencing.

ABSTRACT: Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms.

SUBMITTER: Salipante SJ 

PROVIDER: S-EPMC3838028 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms. ...[more]

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