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Universal labeling of 5'-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity.


ABSTRACT: An artificial RNA ligase specific to RNA with a 5'-triphosphate (PPP-RNA) exhibits broad sequence specificity on model substrates and secondary siRNAs with direct applications in the identification of PPP-RNAs through sequencing.

SUBMITTER: Haugner JC 

PROVIDER: S-EPMC3838195 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Universal labeling of 5'-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity.

Haugner John C JC   Seelig Burckhard B  

Chemical communications (Cambridge, England) 20130801 66


An artificial RNA ligase specific to RNA with a 5'-triphosphate (PPP-RNA) exhibits broad sequence specificity on model substrates and secondary siRNAs with direct applications in the identification of PPP-RNAs through sequencing. ...[more]

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