An efficient strategy for small-scale screening and production of archaeal membrane transport proteins in Escherichia coli.

ABSTRACT:

Background

Membrane proteins play a key role in many fundamental cellular processes such as transport of nutrients, sensing of environmental signals and energy transduction, and account for over 50% of all known drug targets. Despite their importance, structural and functional characterisation of membrane proteins still remains a challenge, partially due to the difficulties in recombinant expression and purification. Therefore the need for development of efficient methods for heterologous production is essential.

Methodology/principal findings

Fifteen integral membrane transport proteins from Archaea were selected as test targets, chosen to represent two superfamilies widespread in all organisms known as the Major Facilitator Superfamily (MFS) and the 5-Helix Inverted Repeat

SUBMITTER: Ma P 

PROVIDER: S-EPMC3838208 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature