Tandem mass spectrometry-based detection of c4'-oxidized abasic sites at specific positions in DNA fragments.

ABSTRACT: Oxidative damage to DNA has been linked to aging, cancer, and other biological processes. Reactive oxygen species and various antitumor agents including bleomycin and ionizing radiation have been shown to cause oxidative DNA sugar damage. Detection of DNA lesions is important for understanding the toxicological or therapeutic consequences associated with such agents. C4'-oxidized abasic sites (C4-AP) are produced by the antitumor drug bleomycin and ionizing radiation. The currently available methods for the detection of C4-AP cannot provide both structural and sequence information. We have developed an LC-ESI-MS-based approach for specific detection and mapping of C4-AP from a mixture of lesions. We show using Fe-bleomycin-damaged DNA that C4-AP can be detected at cytosine and thymine site

SUBMITTER: Chowdhury G 

PROVIDER: S-EPMC3838791 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature