Community-wide assessment of protein-interface modeling suggests improvements to design methodology.

ABSTRACT: The CAPRI (Critical Assessment of Predicted Interactions) and CASP (Critical Assessment of protein Structure Prediction) experiments have demonstrated the power of community-wide tests of methodology in assessing the current state of the art and spurring progress in the very challenging areas of protein docking and structure prediction. We sought to bring the power of community-wide experiments to bear on a very challenging protein design problem that provides a complementary but equally fundamental test of current understanding of protein-binding thermodynamics. We have generated a number of designed protein-protein interfaces with very favorable computed binding energies but which do not appear to be formed in experiments, suggesting that there may be important physical chemistry missing

SUBMITTER: Fleishman SJ 

PROVIDER: S-EPMC3839241 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature