In vivo imaging of human and mouse skin with a handheld dual-axis confocal fluorescence microscope.

ABSTRACT: Advancing molecular therapies for the treatment of skin diseases will require the development of new tools that can reveal spatiotemporal changes in the microanatomy of the skin and associate these changes with the presence of the therapeutic agent. For this purpose, we evaluated a handheld dual-axis confocal (DAC) microscope that is capable of in vivo fluorescence imaging of skin, using both mouse models and human skin. Individual keratinocytes in the epidermis were observed in three-dimensional image stacks after topical administration of near-infrared (NIR) dyes as contrast agents. This suggested that the DAC microscope may have utility in assessing the clinical effects of a small interfering RNA (siRNA)-based therapeutic (TD101) that targets the causative mutation in pachyonychia conge

SUBMITTER: Ra H 

PROVIDER: S-EPMC3839658 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature