Subnuclear relocalization and silencing of a chromosomal region by an ectopic ribosomal DNA repeat.

ABSTRACT: Our research addresses the relationship between subnuclear localization and gene expression in fission yeast. We observed the relocalization of a heterochromatic region, the mating-type region, from its natural location at the spindle-pole body to the immediate vicinity of the nucleolus. Relocalization occurred in response to a DNA rearrangement replacing a boundary element (IR-R) with a ribosomal DNA repeat (rDNA-R). Gene expression was strongly silenced in the relocalized mating-type region through mechanisms that differ from those operating in wild type. Also different from the wild-type situation, programmed recombination events failed to take place in the rDNA-R mutant. Increased silencing and perinucleolar localization depended on Reb1, a DNA-binding protein with cognate sites in the

SUBMITTER: Jakociunas T 

PROVIDER: S-EPMC3839722 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature