CXCL13 mediates prostate cancer cell proliferation through JNK signalling and invasion through ERK activation.

ABSTRACT:

Objectives

  The focus of this study was to determine the dedicator of cytokinesis 2 (DOCK2), extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase-1 (JNK) and Akt signals involved in CXCL13-mediated prostate cancer (PCa) cell invasion and proliferation.

Materials and methods

  Androgen-sensitive (LNCaP), hormone-refractory (PC3) cells and normal cells (RWPE-1) were used to determine CXCL13-mediated PCa cell invasion and proliferation. Immuno-blotting, fast activated cell-based (FACE) ELISA, caspase activity, cell invasion and proliferation assays were performed to ascertain some of the signalling events involved in PCa cell proliferation and invasion.

Results

  Unlike androgen-sensitive LNCaP cells, we report for the first time that the hormone-

SUBMITTER: El-Haibi CP 

PROVIDER: S-EPMC3839818 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature