Feeding low or pharmacological concentrations of zinc oxide changes the hepatic proteome profiles in weaned piglets.

ABSTRACT: Pharmacological levels of zinc oxide can promote growth and health of weaning piglets, but the underlying molecular mechanisms are yet not fully understood. The aim of this study was to determine changes in the global hepatic protein expression in response to dietary zinc oxide in weaned piglets. Nine half-sib piglets were allocated to three dietary zinc treatment groups (50, 150, 2500 mg/kg dry matter). After 14 d, pigs were euthanized and liver samples taken. The increase in hepatic zinc concentration following dietary supplementation of zinc was accompanied by up-regulation of metallothionein mRNA and protein expression. Global hepatic protein profiles were obtained by two-dimensional difference gel electrophoresis following matrix-assisted laser desorption ionization/time-of-flight mas

SUBMITTER: Bondzio A 

PROVIDER: S-EPMC3839893 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature