Protein expression profile of rat type two alveolar epithelial cells during hyperoxic stress and recovery.

ABSTRACT: In rodent model systems, the sequential changes in lung morphology resulting from hyperoxic injury are well characterized and are similar to changes in human acute respiratory distress syndrome. In the injured lung, alveolar type two (AT2) epithelial cells play a critical role in restoring the normal alveolar structure. Thus characterizing the changes in AT2 cells will provide insights into the mechanisms underpinning the recovery from lung injury. We applied an unbiased systems-level proteomics approach to elucidate molecular mechanisms contributing to lung repair in a rat hyperoxic lung injury model. AT2 cells were isolated from rat lungs at predetermined intervals during hyperoxic injury and recovery. Protein expression profiles were determined by using iTRAQ with tandem mass spectromet

SUBMITTER: Bhargava M 

PROVIDER: S-EPMC3840279 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature