Predominant intragenic methylation is associated with gene expression characteristics in a bivalve mollusc.

ABSTRACT: Characterization of DNA methylation patterns in the Pacific oyster, Crassostrea gigas, indicates that this epigenetic mechanism plays an important functional role in gene regulation and may be involved in the regulation of developmental processes and environmental responses. However, previous studies have been limited to in silico analyses or characterization of DNA methylation at the single gene level. Here, we have employed a genome-wide approach to gain insight into how DNA methylation supports the regulation of the genome in C. gigas. Using a combination of methylation enrichment and high-throughput bisulfite sequencing, we have been able to map methylation at over 2.5 million individual CpG loci. This is the first high-resolution methylome generated for a molluscan species. Results in

SUBMITTER: Gavery MR 

PROVIDER: S-EPMC3840415 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature