A modified TALEN-based system for robust generation of knock-out human pluripotent stem cell lines and disease models.

ABSTRACT:

Background

Transcription activator-like effector nucleases (TALENs) have emerged as a tool for enabling targeted gene editing and disruption in difficult systems, such as human pluripotent stem cells (hPSCs). The modular architecture of TAL effectors theoretically enables targeting of any genomic locus and several cloning systems for custom TALEN assembly have recently been established. However, there is a lack of versatile TALEN expression systems applicable to hPSCs.

Results

Here, we extend an existing TALE assembly system by a dual set of expression vectors for efficient application of TALEN technology in hPSCs. This is characterized by improved TALEN architecture as well as antibiotic resistance and fluorescent reporter cassettes, thus enabling enrichment for transfected

SUBMITTER: Frank S 

PROVIDER: S-EPMC3840567 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature